1CU1
CRYSTAL STRUCTURE OF AN ENZYME COMPLEX FROM HEPATITIS C VIRUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS |
| Synchrotron site | APS |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.360, 110.510, 141.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.500 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.26000 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.670 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (98.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.082 | 0.340 |
| Total number of observations | 601477 * | |
| Number of reflections | 50441 | |
| <I/σ(I)> | 21 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 0.07 (mM) | |
| 2 | 1 | 1 | sodium phosphate | 0.08 (M) | |
| 3 | 1 | 1 | PEG6000 | 16 (%(w/v)) | |
| 4 | 1 | 1 | di-potassium hydrogen phosphate | 32 (mM) | |
| 5 | 1 | 1 | MPD | 8 (%(w/v)) | |
| 6 | 1 | 1 | HEPES | 5 (mM) | |
| 7 | 1 | 1 | 0.2 (M) | ||
| 8 | 1 | 1 | glycerol | 2 (%(v/v)) | |
| 9 | 1 | 1 | beta-mercaptoethanol | 2 (mM) |
