1CU0

T4 LYSOZYME MUTANT I78M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-01-01
Detector	RIGAKU RAXIS
Spacegroup name	P 32 2 1
Unit cell lengths	60.480, 60.480, 97.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.173
Rwork	0.173
RMSD bond length	0.021 *
RMSD bond angle	2.600 *
Data reduction software	DENZO
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.072 *
Number of reflections	10304
<I/σ(I)>	6.6
Completeness [%]	90.0 *	27.3
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol