1CT2
CRYSTAL STRUCTURE OF THE OMTKY3 P1 VARIANT OMTKY3-THR18I IN COMPLEX WITH SGPB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-24 |
| Detector | MAC Science DIP-2000H |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.656, 54.530, 45.589 |
| Unit cell angles | 90.00, 119.24, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.954 |
| Data reduction software | DIP (XPRESS) |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.068 | 0.326 |
| Total number of observations | 68327 * | |
| Number of reflections | 21917 * | |
| <I/σ(I)> | 13.24 | |
| Completeness [%] | 91.5 | 59.9 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | PEG 4000 sodium potassium phosphate , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 4-10 (%) |
