1CSV
REPLACEMENTS IN A CONSERVED LEUCINE CLUSTER IN THE HYDROPHOBIC HEME POCKET OF CYTOCHROME C
Experimental procedure
Spacegroup name | P 43 21 2 |
Unit cell lengths | 36.550, 36.550, 138.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.900 |
R-factor | 0.192 |
RMSD bond length | 0.019 |
RMSD bond angle | 0.040 |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.900 * |
Total number of observations | 8072 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.7 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 75 (%sat) | |
3 | 1 | drop | sodium phosphate | 0.1 (M) | |
4 | 1 | reservoir | ammonium sulfate | 90 (%sat) |