1CSJ

CRYSTAL STRUCTURE OF THE RNA-DEPENDENT RNA POLYMERASE OF HEPATITIS C VIRUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	105
Detector technology	CCD
Collection date	1999-05-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	67.063, 96.895, 194.432
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.000 - 2.800
R-factor	0.237 *
Rwork	0.237
R-free	0.28600
RMSD bond length	0.008
RMSD bond angle	1.450
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.073	0.127
Number of reflections	31327
<I/σ(I)>	15.3
Completeness [%]	97.8	85.9
Redundancy	3.5	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	5% (w/v) PEG 8000, 0.1 M sodium citrate, 5% 2-propanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	drop	dithiothreitol	20 (mM)
4	1	drop	EDTA	1 (mM)
5	1	reservoir	PEG8000	5 (%(w/v))
6	1	reservoir	sodium citrate	0.1 (M)
7	1	reservoir	2-propanol	5 (%(v/v))