1CSG

Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	46.600, 58.800, 126.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.700
R-factor	0.254
Rwork	0.254
RMSD bond length	0.013
RMSD bond angle	3.260
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.079 *
Total number of observations	35397 *
Number of reflections	9083 *
Completeness [%]	90.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	4 *	referred to J.Biol.Chem.265.452-453 1990 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	PEG8000	10 (%)
3	1	drop	sodium phosphate	16 (M)
4	1	reservoir	PEG8000	20 (%)
5	1	reservoir	sodium phosphate	16 (mM)