1CS7
SYNTHETIC DNA HAIRPIN WITH STILBENEDIETHER LINKER
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-02-06 |
Detector | APS-1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.760, 67.890, 61.830 |
Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
Resolution | 8.000 - 3.200 |
R-factor | 0.257 |
Rwork | 0.257 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.710 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.260 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.097 | 0.095 |
Number of reflections | 3434 | |
Completeness [%] | 97.1 | 99 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 298 | OLIGONUCLEOTIDE, SRCL2, NA-CACODYLATE BUFFER, 2-METHYL-2,4-PENTANEDIOL, pH 6.3, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SRCL2 | ||
2 | 1 | 1 | SODIUM CACODYLATE BUFFER | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.8 (mM) | |
2 | 1 | drop | sodium cacodylate | 40 (mM) | |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | reservoir | 40 (%) |