1CS3

STRUCTURE OF BTB/POZ TRANSCRIPTION REPRESSION DOMAIN FROM PROMELOCYTIC LEUKEMIA ZINC FINGER ONCOPROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	190
Detector technology	IMAGE PLATE
Collection date	1997-10-03
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.9879,0.9795,0.9791, 0.9667
Spacegroup name	I 2 2 2
Unit cell lengths	38.800, 78.200, 85.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.228
Rwork	0.228
R-free	0.27200
RMSD bond length	0.008
RMSD bond angle	1.090
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.060
High resolution limit [Å]	2.000	2.000
Rmerge	0.070
Total number of observations	115110 *
Number of reflections	8790
<I/σ(I)>	35.5	24
Completeness [%]	96.7	95
Redundancy	13	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8		reservoir contains two times the concentration of salts, buffer, and precipitating agent *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	isopropanol	8 (%)
3	1	drop		600 (mM)
4	1	drop	Tris-HCl	50 (mM)
5	1	drop	HEPES	50 (mM)