1CRX
CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10-15 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 107.700, 121.000, 180.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.208 * |
Rwork | 0.201 |
R-free | 0.26300 |
Structure solution method | MIR, MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.850 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.470 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.069 * | |
Number of reflections | 42896 | |
<I/σ(I)> | 20 | 10 |
Completeness [%] | 93.0 | 87 |
Redundancy | 5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 | 18 * | 100 MM ACETATE, PH 5 24% MPD 20 MM CACL2, pH 5.0 |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ACETATE | ||
2 | 1 | 1 | CACL2 | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium acetate | 50 (mM) | |
2 | 1 | drop | MPD | 12 (%) | |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | Cre | 0.050 (mM) | |
5 | 1 | drop | loxA | 0.075 (mM) | |
6 | 1 | reservoir | acetate | 100 (mM) | |
7 | 1 | reservoir | MPD | 24 (%) | |
8 | 1 | reservoir | 20 (mM) |