1CRB
CRYSTALLOGRAPHIC STUDIES ON A FAMILY OF CELLULAR LIPOPHILIC TRANSPORT PROTEINS. REFINEMENT OF P2 MYELIN PROTEIN AND THE STRUCTURE DETERMINATION AND REFINEMENT OF CELLULAR RETINOL-BINDING PROTEIN IN COMPLEX WITH ALL-TRANS-RETINOL
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.090, 47.390, 69.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.24800 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.310 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 * |
| Completeness [%] | 80.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 75 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 30 (mM) | |
| 4 | 1 | reservoir | PEG6000 | 20 (%) | |
| 5 | 1 | reservoir | 0.1-20 (mM) |
