1CR9
CRYSTAL STRUCTURE OF THE ANTI-PRION FAB 3F4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10-30 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.900, 77.600, 70.600 |
| Unit cell angles | 90.00, 110.56, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.171 * |
| Rwork | 0.171 |
| R-free | 0.21700 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.782 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 | 0.220 |
| Number of reflections | 33314 * | 2242 * |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 98.5 | 90.6 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | ammonium sulphate, PEG 400, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MOPS | 10 (mM) | |
| 2 | 1 | drop | protein | 20 (mg/ml) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | 2 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 6 | 1 | reservoir | PEG400 | 2 (%(v/v)) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) |
