1CR7
PEANUT LECTIN-LACTOSE COMPLEX MONOCLINIC FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 128.320, 126.820, 85.610 |
| Unit cell angles | 90.00, 116.14, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.212 |
| Rwork | 0.207 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pel |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.600 * |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.600 | |
| Rmerge | 0.134 | 0.316 * |
| Total number of observations | 311508 * | |
| Number of reflections | 65505 | |
| Completeness [%] | 86.3 | 68.4 * |
| Redundancy | 4.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.4 * | 20 * | Salunke, D.M., (1983) FEBS Lett., 156, 127. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | phosphate | ||
| 3 | 1 | drop | PEG6000 | 40 (%(w/v)) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | sodium acetate | 0.05 (M) | |
| 6 | 1 | reservoir | lactose | 1.5 (mM) |
