1CR7

PEANUT LECTIN-LACTOSE COMPLEX MONOCLINIC FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	128.320, 126.820, 85.610
Unit cell angles	90.00, 116.14, 90.00

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.212
Rwork	0.207
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pel
RMSD bond length	0.010
RMSD bond angle	27.600 *
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.600
Rmerge	0.134	0.316 *
Total number of observations	311508 *
Number of reflections	65505
Completeness [%]	86.3	68.4 *
Redundancy	4.75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.4 *	20 *	Salunke, D.M., (1983) FEBS Lett., 156, 127. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop	phosphate
3	1	drop	PEG6000	40 (%(w/v))
4	1	reservoir		0.2 (M)
5	1	reservoir	sodium acetate	0.05 (M)
6	1	reservoir	lactose	1.5 (mM)