1CQR
CRYSTAL STRUCTURE OF THE STROMELYSIN CATALYTIC DOMAIN AT 2.0 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-24 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.200, 79.100, 107.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.000 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.26600 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.210 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 12.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | 0.058 |
| Number of reflections | 21471 | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 94.7 | 94.7 |
| Redundancy | 4.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 298 | 20-24% PEG 8000, 2.5% 2-propanol, 10mM CaCl2, 0.1 M Tris-HCL buffer, VAPOR DIFFUSION, HANGING DROP, temperature 298.K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 20-24 (%(w/v)) | |
| 3 | 1 | reservoir | 2-propanol | 2.5 (%(v/v)) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) |
