1CQK

CRYSTAL STRUCTURE OF THE CH3 DOMAIN FROM THE MAK33 ANTIBODY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	20
Detector technology	IMAGE PLATE
Collection date	1998-07-14
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	48.700, 42.800, 50.400
Unit cell angles	90.00, 105.93, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.205 *
Rwork	0.196
R-free	0.24860
RMSD bond length	0.007
RMSD bond angle	1.350
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.104	0.273
Total number of observations	57677 *
Number of reflections	10340
<I/σ(I)>	10.1
Completeness [%]	93.4	83.6
Redundancy	5.58	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	Tris, ammonium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 18K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	CH3	10 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir	ammonium sulfate	2.0 (M)