1CQJ
CRYSTAL STRUCTURE OF DEPHOSPHORYLATED E. COLI SUCCINYL-COA SYNTHETASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 1993-12-04 |
Detector | FUJI |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 98.820, 98.820, 404.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.178 |
Rwork | 0.172 |
R-free | 0.22800 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.900 |
Data reduction software | WEIS |
Data scaling software | SCALKB2 |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.092 | 0.350 |
Total number of observations | 258739 * | |
Number of reflections | 45002 | |
Completeness [%] | 98.7 * | 96.6 |
Redundancy | 5.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.3 | 21 * | ammonium sulfate, potassium phosphate, coenzyme A, pH 7.3, MICRODIALYSIS, temperature 294K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | ammonium sulfate | 1.9 (M) | |
2 | 1 | 1 | potassium phosphate | 0.1 (M) | |
3 | 1 | 1 | dithiothreitol | 1 (mM) | |
4 | 1 | 1 | CoA | 0.1 (mM) | |
5 | 1 | 1 | EDTA | 0.1 (mM) |