1CQ7
ASPARTATE AMINOTRANSFERASE (E.C. 2.6.1.1) COMPLEXED WITH C5-PYRIDOXAL-5P-PHOSPHATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1998-09-09 |
Detector | RIGAKU RAXIS |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 157.580, 85.590, 78.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.400 |
R-factor | 0.215 * |
Rwork | 0.215 |
R-free | 0.25100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.096 | 0.317 |
Total number of observations | 91223 * | |
Number of reflections | 20972 | |
<I/σ(I)> | 7.5 | |
Completeness [%] | 98.5 | 97.1 |
Redundancy | 4.3 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | ammonium sulfate, potassium phosphate, C4-pyridoxal-5p-phosphate,sodium azide , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 10 (mM) | |
3 | 1 | drop | PLP-amino acid | 0.010 (mM) | |
4 | 1 | drop | 0.3 (mM) | pH8.0 | |
5 | 1 | reservoir | ammonium sulfate | 35 (%sat) | |
6 | 1 | reservoir | potassium phosphate | 10 (mM) |