1CQ6

ASPARTATE AMINOTRANSFERASE COMPLEX WITH C4-PYRIDOXAL-5P-PHOSPHATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-09-05
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 2 2 21
Unit cell lengths	157.150, 86.320, 79.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.241 *
Rwork	0.241
R-free	0.29700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.087	0.220
Total number of observations	79877 *
Number of reflections	14620
<I/σ(I)>	8.2
Completeness [%]	96.2	95.5
Redundancy	5.5	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	20 *	ammonium sulfate, potassium phosphate, C4-pyridoxal-5p-phosphate, sodium azide , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	potassium phosphate	10 (mM)
3	1	drop	PLP-amino acid	0.010 (mM)
4	1	drop		0.3 (mM)	pH8.0
5	1	reservoir	ammonium sulfate	35 (%sat)
6	1	reservoir	potassium phosphate	10 (mM)