1CPY

SITE-DIRECTED MUTAGENESIS ON (SERINE) CARBOXYPEPTIDASE Y FROM YEAST. THE SIGNIFICANCE OF THR 60 AND MET 398 IN HYDROLYSIS AND AMINOLYSIS REACTIONS

Experimental procedure

Spacegroup name	P 21 3
Unit cell lengths	112.000, 112.000, 112.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.600
R-factor	0.18
RMSD bond length	0.023
RMSD bond angle	3.300
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.600 *
Rmerge	0.072 *
Total number of observations	48636 *
Number of reflections	14110 *
Completeness [%]	92.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		Endrizzi, J.A., (1994) Biochemistry, 33, 11106. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	18-24 (%)
2	1	reservoir		0.3 (M)
3	1	reservoir		0.05 (M)
4	1	reservoir	imidazole/NaOH	100 (mM)
5	1	drop	CPD-Y	10 (mg/ml)
6	1	drop		0.1 (M)
7	1	drop	DTT	1 (mM)
8	1	drop	citrate/NaOH	20 (mM)