1CPQ

CYTOCHROME C' FROM RHODOPSEUDOMONAS CAPSULATA

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1994-04-26
Detector	FUJI
Spacegroup name	P 21 21 2
Unit cell lengths	47.820, 72.590, 34.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.720
R-factor	0.15
Rwork	0.150
Structure solution method	MOLECULAR
Starting model (for MR)	RHODOSPIRILLUM RUBRUM CYTOCHROME C
RMSD bond length	0.010
RMSD bond angle	19.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.700
Rmerge	0.071
Total number of observations	52922 *
Number of reflections	11127
<I/σ(I)>	12.4
Completeness [%]	80.2
Redundancy	3.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium sulfate	100 (mM)
3	1	reservoir	ammonium sulfate	200 (mM)
4	1	reservoir	PEG8000	30 (%(w/v))