1COZ
CTP:GLYCEROL-3-PHOSPHATE CYTIDYLYLTRANSFERASE FROM BACILLUS SUBTILIS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 277 |
Detector technology | AREA DETECTOR |
Detector | SDMS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.500, 61.400, 56.400 |
Unit cell angles | 90.00, 113.10, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.25500 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 23.400 * |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.061 * | 0.125 * |
Total number of observations | 45342 * | |
Number of reflections | 16917 | |
Completeness [%] | 88.6 | 83.8 * |
Redundancy | 2.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | 100 MM TRIS PH 8.5 200 MM LITHIUM SULFATE 30% PEG 3350 (BAKER) |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6.3 (mg/ml) | |
2 | 1 | drop | Tris | 55 (mM) | |
3 | 1 | drop | EDTA | 0.63 (mM) | |
4 | 1 | drop | dithiothreitol | 0.63 (mM) | |
5 | 1 | drop | PEG3350 | 11 (%) | |
6 | 1 | drop | lithium sulfate | 75 (mM) | |
7 | 1 | reservoir | PEG3350 | 30 (%) | |
8 | 1 | reservoir | lithium sulfate | 200 (mM) | |
9 | 1 | reservoir | Tris | 100 (mM) |