1COS

CRYSTAL STRUCTURE OF A SYNTHETIC TRIPLE-STRANDED ALPHA-HELICAL BUNDLE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	27.700, 38.700, 77.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.100
R-factor	0.18 *
Rwork	0.180
RMSD bond length	0.017
RMSD bond angle	2.600
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.057 *
Number of reflections	4512 *
Completeness [%]	86.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	22 *	referred to 'Lovejoy, B.', (1992) 'Protein Sci.', 1, 956. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	2 (mg/ml)
2	1	reservoir	potassium phosphate-sodium hydroxide	0.05 (M)
3	1	reservoir	ammonium sulfate	2.95 (M)