1COL
REFINED STRUCTURE OF THE PORE-FORMING DOMAIN OF COLICIN A AT 2.4 ANGSTROMS RESOLUTION
Experimental procedure
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.000, 73.000, 171.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.500 * |
R-factor | 0.26 * |
RMSD bond length | 0.014 |
RMSD bond angle | 0.049 |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 6.000 * |
High resolution limit [Å] | 2.500 * |
Number of reflections | 10024 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | referred to J.Mol.Biol. 190.133-134 1986 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | dropg | protein | 14 (mg/ml) | |
2 | 1 | drop | citrate | 50 (mM) | can be replaced with succinate |
3 | 1 | drop | ammonium sulfate | 2.3 (M) | |
4 | 1 | reservoir | citrate | 50 (mM) | can be replaced with succinate |
5 | 1 | reservoir | ammonium sulfate | 2.3 (M) |