1COL

REFINED STRUCTURE OF THE PORE-FORMING DOMAIN OF COLICIN A AT 2.4 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	73.000, 73.000, 171.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500 *
R-factor	0.26 *
RMSD bond length	0.014
RMSD bond angle	0.049
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	2.500 *
Number of reflections	10024 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			referred to J.Mol.Biol. 190.133-134 1986 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	dropg	protein	14 (mg/ml)
2	1	drop	citrate	50 (mM)	can be replaced with succinate
3	1	drop	ammonium sulfate	2.3 (M)
4	1	reservoir	citrate	50 (mM)	can be replaced with succinate
5	1	reservoir	ammonium sulfate	2.3 (M)