1CO7
R117H mutant rat anionic trypsin complexed with bovine pancreatic trypsin inhibitor (BPTI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 92.696, 92.696, 62.406 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tgi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.580 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 22249 | |
| <I/σ(I)> | 11.7 | 2.2 |
| Completeness [%] | 90.0 | 89.5 |
| Redundancy | 5.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.5 |
