1CO6
CRYSTAL STRUCTURE OF FERROCYTOCHROME C2 FROM RHODOPSEUDOMONAS VIRIDIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 291 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1994-07-15 |
| Detector | WEISSENBERG |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 76.130, 76.130, 40.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.600 |
| Rwork | 0.182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cyt |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.037 |
| Data reduction software | WEIS |
| Data scaling software | PROTEIN |
| Phasing software | PHASER |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.072 |
| Number of reflections | 16166 |
| Completeness [%] | 89.1 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 | 4 * | CRYSTALS WERE GROWN FROM AN AMMONIUM SULFATE SOLUTION AT PH 8.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 3 | 1 | drop | ammonium sulfate | 40 (%sat) | |
| 4 | 1 | reservoir | ammonium sulfate | 70 (%sat) |
