1CNM
ENHANCEMENT OF CATALYTIC EFFICIENCY OF PROTEINASE K THROUGH EXPOSURE TO ANHYDROUS ORGANIC SOLVENT AT 70 DEGREES CELSIUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 285 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.320, 68.320, 108.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| R-factor | 0.168 * |
| Rwork | 0.168 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2prk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.000 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.045 | 0.087 |
| Total number of observations | 89243 * | |
| Number of reflections | 12156 | |
| <I/σ(I)> | 40.01 | 24.41 |
| Completeness [%] | 97.0 | 94 |
| Redundancy | 5.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 6.5 * | 4 * | CRYSTALLIZED BY DISSOLVING THE 10%(W/V) OF LYOPHILIZED ENZYME IN 50MM TRIS.HCL, 1MM CACL2, PH 6.5. 25 MICRO LITRE DROPS OF THIS SOLUTION WERE EQUILIBRATED IN A MICRODIALYSIS SETUP AGAINST 1M NANO3 IN THE SAME BUFFER AT 4 DEGREE., pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | enzyme | 10 (%(w/v)) | |
| 2 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 3 | 1 | 1 | 1 (mM) | ||
| 4 | 1 | 2 | 1 (M) |
