1CNK

X-RAY CRYSTALLOGRAPHIC STUDIES OF ENGINEERED HYDROGEN BOND NETWORKS IN A PROTEIN-ZINC BINDING SITE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-06-23
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	42.700, 41.700, 73.000
Unit cell angles	90.00, 104.60, 90.00

Refinement procedure

Resolution	8.000 - 2.150
R-factor	0.176
Rwork	0.176
RMSD bond length	0.011
RMSD bond angle	25.900 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.150 *
Rmerge	0.067
Number of reflections	18287
Completeness [%]	92.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-Cl	50 (mM)	or Tris-SO4, pH8.0
2	1	reservoir	ammonium sulfate	50-70 (%saturated)	1.75-2.5M
3	1	drop	protein	0.3 (mM)
4	1	drop	Tris-Cl	50 (mM)	or Tris-SO4, pH8.0