1CNH
X-RAY CRYSTALLOGRAPHIC STUDIES OF ENGINEERED HYDROGEN BOND NETWORKS IN A PROTEIN-ZINC BINDING SITE
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1993-10-10 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.700, 41.700, 73.000 |
| Unit cell angles | 90.00, 104.60, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.050 |
| R-factor | 0.158 |
| Rwork | 0.158 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.500 * |
| Data reduction software | BUDDHA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 * |
| High resolution limit [Å] | 2.050 * |
| Rmerge | 0.071 |
| Total number of observations | 29234 * |
| Number of reflections | 15319 |
| Completeness [%] | 87.0 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-Cl | 50 (mM) | or Tris-SO4, pH8.0 |
| 2 | 1 | reservoir | ammonium sulfate | 50-70 (%saturated) | 1.75-2.5M |
| 3 | 1 | drop | protein | 0.3 (mM) | |
| 4 | 1 | drop | Tris-Cl | 50 (mM) | or Tris-SO4, pH8.0 |
