Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-04 |
| Detector | ADSC |
| Wavelength(s) | 0.9792, 0.9790, 0.964 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 67.200, 67.200, 217.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.240 |
| R-free | 0.28300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (CNS) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.047 | 0.209 |
| Number of reflections | 9052 | |
| <I/σ(I)> | 8.8 | 3.5 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 6.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | pH 8.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | reservoir | mPEG2000 | 16 (%) | |
| 5 | 1 | reservoir | MPD | 10 (%) | |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) |
