1CMA
MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Detector technology | OSCILLATION CAMERA |
Detector | ARNDT-WONACOTT |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 121.350, 121.350, 84.810 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.800 |
R-factor | 0.22 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.680 |
Refinement software | TNT |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.800 * |
Rmerge | 0.074 * |
Total number of observations | 33481 * |
Number of reflections | 8856 |
Completeness [%] | 97.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
10 | 1 | 2 | NA CACODYLATE | ||
11 | 1 | 2 | NA AZIDE | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | NA AZIDE | ||
5 | 1 | 1 | CACL2 | ||
6 | 1 | 1 | NACL | ||
7 | 1 | 1 | SAM | ||
8 | 1 | 2 | WATER | ||
9 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | oligonucleotide | 5 (mg/ml) | |
2 | 1 | drop | protein | 9 (mg/ml) | |
3 | 1 | drop | calcium chloride | 22 (mM) | |
4 | 1 | drop | sodium cacodylate | 10 (mM) | |
5 | 1 | drop | sodium chloride | 6 (mM) | |
6 | 1 | drop | SAM | 1 (mg/ml) | |
7 | 1 | reservoir | sodium cacodylate | 10 (mM) | |
8 | 1 | reservoir | sodium azaid | 1 (mM) | |
9 | 1 | reservoir | 35 (%(v/v)) |