1CM5
CRYSTAL STRUCTURE OF C418A,C419A MUTANT OF PFL FROM E.COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 159.059, 159.059, 159.784 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25100 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.300 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.070 |
| Total number of observations | 469884 * |
| Number of reflections | 87117 |
| Completeness [%] | 95.6 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.3 | pH 7.3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 10 | 1 | 2 | EDTA | 1 (mM) | |
| 11 | 1 | 2 | 3 (mM) | ||
| 12 | 1 | 2 | 2 (mM) | ||
| 13 | 1 | 2 | PEG1000 | 24-30 (%(w/v)) | |
| 2 | 1 | 1 | Mops/NH3 | 25 (mM) | |
| 3 | 1 | 1 | 50 (mM) | ||
| 4 | 1 | 1 | dithiothreitol | 10 (mM) | |
| 5 | 1 | 1 | 3 (mM) | ||
| 6 | 1 | 1 | PEG1000 | 16 (%(w/v)) | |
| 7 | 1 | 2 | Mops/NH3 | 25 (mM) | |
| 8 | 1 | 2 | 50 (mM) | ||
| 9 | 1 | 2 | dithiothreitol | 1 (mM) |
