1CM3
HIS15ASP HPR FROM E. COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 1994-06-21 |
Detector | BRANDEIS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.570, 45.340, 27.620 |
Unit cell angles | 90.00, 104.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.600 |
R-factor | 0.191 |
Rwork | 0.191 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | WILD TYPE E. COLI HPR |
RMSD bond length | 0.001 |
RMSD bond angle | 2.400 |
Data reduction software | DENZO |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 1.600 |
Number of reflections | 10525 |
Completeness [%] | 93.4 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.4 * | 14 * | pH 5.4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | citrate phosphate | 0.1 (M) | |
2 | 1 | reservoir | ammonium sulfate | 20-25 (%sat) |