1CLZ

IGG FAB (IGG3, KAPPA) FRAGMENT (MBR96) COMPLEXED WITH LEWIS Y NONOATE METHYL ESTER

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1993-01
Detector	SIEMENS-NICOLET X100
Spacegroup name	P 21 21 21
Unit cell lengths	69.400, 84.900, 86.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.780 *
R-factor	0.197
Rwork	0.197
R-free	0.29500
RMSD bond length	0.008
RMSD bond angle	27.500 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.100	2.950
High resolution limit [Å]	2.780	2.780
Rmerge	0.070
Total number of observations	23962 *
Number of reflections	9937
<I/σ(I)>	12.6	3.8
Completeness [%]	73.9	43.8
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		Chang, C.Y., (1994) J.Mol.Biol., 235, 372. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	imidazole-HCl	10 (mM)
2	1	drop	protein	8 (mg/ml)
3	1	reservoir	sodium formate	4 (M)
4	1	reservoir		1 (mM)