1CLY

IGG FAB (HUMAN IGG1, KAPPA) CHIMERIC FRAGMENT (CBR96) COMPLEXED WITH LEWIS Y NONOATE METHYL ESTER

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1993-07
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	81.700, 81.700, 166.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.238
Rwork	0.238
R-free	0.29800
RMSD bond length	0.009
RMSD bond angle	27.100 *
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.800	2.750
High resolution limit [Å]	2.500 *	2.510
Rmerge	0.059	0.305
Total number of observations	59769 *
Number of reflections	20051
<I/σ(I)>	11.9	2
Completeness [%]	91.7	90.8
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.6 *		Chang, C.Y., (1994) J.Mol.Biol., 235, 372. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	0.1 (M)	pH4.6
2	1	reservoir	ammonium sulfate	0.1 (M)
3	1	reservoir	PEG8000	13-15 (%)