1CLX

CATALYTIC CORE OF XYLANASE A

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1993-01-11
Spacegroup name	P 21 21 21
Unit cell lengths	96.110, 97.320, 151.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.166 *
Rwork	0.166
RMSD bond length	0.008 *
RMSD bond angle	0.027 *
Data reduction software	DENZO
Refinement software	RESTRAIN

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	6.920 *
High resolution limit [Å]	1.790 *	1.790 *
Rmerge	0.077	0.479 *
Total number of observations	703625 *
Number of reflections	105167	7963 *
Completeness [%]	78.8	61.5 *
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	23.3 (mg/ml)
2	1	drop	PEG3400	2.2-2.7 (%)
3	1	drop	phosphate	66.7 (mM)
4	1	reservoir	PEG3400	10-12 (%)
5	1	reservoir	phosphate	200 (mM)