1CLU
H-RAS COMPLEXED WITH DIAMINOBENZOPHENONE-BETA,GAMMA-IMIDO-GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-18 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-04-09 |
| Detector | SIEMENS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 40.200, 40.200, 161.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 - 1.700 |
| R-factor | 0.203 * |
| Rwork | 0.203 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p21 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 26.580 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.084 | 0.260 |
| Total number of observations | 151581 * | |
| Number of reflections | 17463 * | |
| <I/σ(I)> | 15.2 | 2.5 |
| Completeness [%] | 99.0 | 95.3 |
| Redundancy | 8.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 | SEE REFERENCE DECRIBING THE STRUCTURE AND CITATIONS THEREIN, pH 7.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 3-4 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 64 (mM) | |
| 3 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 4 | 1 | 1 | 10 (mM) | ||
| 5 | 1 | 1 | sodium azide | 1 (mM) | |
| 6 | 1 | 1 | PEG1450 | 20 (%(w/v)) |
