1CLQ
CRYSTAL STRUCTURE OF A REPLICATION FORK DNA POLYMERASE EDITING COMPLEX AT 2.7 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-10-15 |
| Detector | ADSC |
| Spacegroup name | H 3 |
| Unit cell lengths | 209.740, 209.740, 114.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.700 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1waj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.080 * |
| Total number of observations | 51455 * |
| Number of reflections | 23329 * |
| <I/σ(I)> | 18.1 |
| Completeness [%] | 97.2 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 12 * | 12% (W/V) PEG 350MME, 150 MM CACL2, 100 MM SODIUM CACODYLATE, PH 6.5, 12 DEGREES C, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG | ||
| 2 | 1 | 1 | CACL2 | ||
| 3 | 1 | 1 | SODIUM CACODYLATE | ||
| 4 | 1 | 2 | PEG |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | mPEG350 | 12 (%(w/v)) | |
| 2 | 1 | reservoir | calcium chloride | 150 (mM) | |
| 3 | 1 | reservoir | sodium cacodylate | 100 (mM) |
