1CLO

ANTI-CARCINOEMBRYONIC ANTIGEN MONOCLONAL ANTIBODY A5B7

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-06-20
Detector	MAR scanner 180 mm plate
Spacegroup name	P 1 21 1
Unit cell lengths	38.890, 79.680, 68.520
Unit cell angles	90.00, 104.91, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.212
Rwork	0.212
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.000	2.000
Rmerge	0.047	0.115
Total number of observations	95102 *
Number of reflections	27439
<I/σ(I)>	24.1	9.7
Completeness [%]	99.2	98
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *		Banfield, M.J., (1996) Acta Cryst. D., 52, 1107. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Bis-Tris	20 (mM)
3	1	reservoir	PEG4000	17-21 (%)
4	1	reservoir	sodium acetate	50 (mM)