1CLM
STRUCTURE OF PARAMECIUM TETRAURELIA CALMODULIN AT 1.8 ANGSTROMS RESOLUTION
Experimental procedure
Spacegroup name | P 1 |
Unit cell lengths | 29.660, 53.790, 25.490 |
Unit cell angles | 92.84, 97.02, 88.54 |
Refinement procedure
Resolution | 10.000 * - 1.800 |
R-factor | 0.21 |
Rwork | 0.210 |
RMSD bond length | 0.016 |
RMSD bond angle | 3.000 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.800 * |
Rmerge | 0.070 * |
Total number of observations | 53853 * |
Number of reflections | 11458 * |
Completeness [%] | 85.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | calmodulin | 3625 (mg/ml) | |
2 | 1 | drop | distilled water | 1 ml | |
3 | 1 | drop | 5 (mM) | 1 ml | |
4 | 1 | drop | MPD | 55 (%(v/v)) | 2 ml |
5 | 1 | drop | cacodylate | ||
6 | 1 | reservoir | MPD | 55 (%(v/v)) | |
7 | 1 | reservoir | cacodylate | 0.05 (M) |