1CLA
EVIDENCE FOR TRANSITION-STATE STABILIZATION BY SERINE-148 IN THE CATALYTIC MECHANISM OF CHLORAMPHENICOL ACETYLTRANSFERASE
Experimental procedure
| Spacegroup name | H 3 2 |
| Unit cell lengths | 107.340, 107.340, 123.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.340 |
| R-factor | 0.172 |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 0.043 * |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.340 * |
| Rmerge | 0.033 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 6.3 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | buttom | protein | 5 (mg/ml) | |
| 2 | 1 | buttom | MES | 10 (mM) | |
| 3 | 1 | reservoir | MPD | 4 (%) | |
| 4 | 1 | reservoir | MES | 10 (mM) | |
| 5 | 1 | reservoir | chloramphenicol | 1 (mM) | |
| 6 | 1 | reservoir | hexa amminecobalt chloride | 0.5 (mM) |
