1CL8
A PRE-TRANSITION STATE ECO RI ENDONUCLEASE/COGNATE DNA (TCGCGAPTTCGCG) COMPLEX WITH DNA BASE ANALOG PURINE (P)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 117.833, 117.833, 48.989 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.28700 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1ckq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.067 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.105 | 0.432 |
| Number of reflections | 35192 | |
| <I/σ(I)> | 19.3 | 2.6 |
| Completeness [%] | 98.3 | 91 |
| Redundancy | 10.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | pH 7.5, VAPOR DIFFUSION, HANGING DROP |
