1CL1
CYSTATHIONINE BETA-LYASE (CBL) FROM ESCHERICHIA COLI
Experimental procedure
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1995-04-10 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.900, 154.700, 152.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.830 |
R-factor | 0.151 |
Rwork | 0.151 |
R-free | 0.22100 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.760 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.870 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.042 | 0.210 |
Total number of observations | 152322 * | |
Number of reflections | 58549 * | |
Completeness [%] | 92.6 | 95 |
Redundancy | 3.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.3 * | 20% PEG400, 0.15M CACL2, 0.1 M HEPES/NAOH (PH 8.2) |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 5 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 10-14 (%) | |
3 | 1 | reservoir | HEPES | 100 (mM) | pH7.3 |
4 | 1 | reservoir | 150 (mM) |