1CKP

HUMAN CYCLIN DEPENDENT KINASE 2 COMPLEXED WITH THE INHIBITOR PURVALANOL B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-02-15
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	53.550, 71.350, 72.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.000 - 2.050
R-factor	0.192
Rwork	0.192
R-free	0.26000
Structure solution method	OTHER
Starting model (for MR)	1hck
RMSD bond length	0.007
RMSD bond angle	23.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	2.110
High resolution limit [Å]	2.050	2.050
Rmerge	0.055	0.185
Number of reflections	17655
<I/σ(I)>	13.3
Completeness [%]	98.7	91.6
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.4		pH 7.4