1CKF

T52A MUTANT HUMAN LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1998-03-01
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	56.510, 61.250, 33.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
Rwork	0.170
Structure solution method	OTHER
Starting model (for MR)	WILD-TYPE OF HUMAN LYSOZYME
RMSD bond length	0.009
RMSD bond angle	23.630 *
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.075	0.192
Total number of observations	22975 *
Number of reflections	9611
<I/σ(I)>		3.4
Completeness [%]	84.1	50
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5		protein solution is mixed in a 1:1 ratio with well solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir		2.5 (M)
3	1	reservoir	acetate	20 (mM)