1CK4
CRYSTAL STRUCTURE OF RAT A1B1 INTEGRIN I-DOMAIN.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.770, 85.920, 132.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.200 |
| R-factor | 0.234 * |
| Rwork | 0.234 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aox |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | |
| High resolution limit [Å] | 2.200 | 2.200 * |
| Rmerge | 0.056 | |
| Number of reflections | 19238 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 91.0 | 77.6 * |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | |
| 2 | 1 | drop | 2-mercaptoethanol | 10 (mM) | |
| 3 | 1 | drop | protein | 11 (mg/ml) | |
| 4 | 1 | reservoir | PEG8000 | 25 (%(w/v)) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 6 | 1 | reservoir | sodium acetate | 0.2 (M) |
