1CJY
HUMAN CYTOSOLIC PHOSPHOLIPASE A2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-03-01 |
| Detector | ADSC |
| Wavelength(s) | 1.2, 1.6496 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 153.590, 95.490, 139.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.500 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.29800 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.940 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | |
| High resolution limit [Å] | 2.500 | |
| Rmerge | 0.064 * | 0.300 * |
| Total number of observations | 271686 * | |
| Number of reflections | 66223 | |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 93.3 | 87.9 * |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.2 * | 18 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 0.1 (M) | |
| 2 | 1 | drop | spermidine | 30 (mM) | |
| 3 | 1 | drop | PEG1000 | 8 (%) | |
| 4 | 1 | drop | DMSO | 13 (%) | |
| 5 | 1 | drop | protain | 12 (mg/ml) |
