1CJX
CRYSTAL STRUCTURE OF PSEUDOMONAS FLUORESCENS HPPD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-06-01 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.590, 142.750, 159.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.219 * |
| Rwork | 0.219 |
| R-free | 0.27600 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.031 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 * | |
| Total number of observations | 283368 * | |
| Number of reflections | 64393 | |
| <I/σ(I)> | 41.67 | |
| Completeness [%] | 93.2 | 86.7 |
| Redundancy | 4.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 18-25 % PEG 4000 0.2 M AMMONIUM ACETATE, 0.1 M CITRATE, PH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 18-25 (%) | |
| 2 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | trisodium citrate | 0.1 (M) | |
| 4 | 1 | drop | protein | 10-13 (mg/ml) |
