1CJQ
X-RAY CRYSTALLOGRAPHIC STUDIES OF THE DENATURATION OF THE DENATURATION OF RIBONUCLEASE S.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 44.800, 44.800, 98.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.175 * |
| Rwork | 0.175 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rnv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.200 |
| Data reduction software | AUTOMAR |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | |
| Number of reflections | 2233 | |
| Completeness [%] | 76.6 | 49.3 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.5 * | Kim, E.E., (1992) Biochemistry, 31, 12304. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12.5 (mg/ml) | |
| 2 | 1 | drop | 6.0 (M) | ||
| 3 | 1 | drop | sodium acetate | 0.1 (M) | |
| 4 | 1 | reservoir | 80 (%sat) | ||
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
