1CJP

CONCANAVALIN A COMPLEX WITH 4'-METHYLUMBELLIFERYL-ALPHA-D-GLUCOPYRANOSIDE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-11-30
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	81.620, 128.710, 82.230
Unit cell angles	90.00, 118.47, 90.00

Refinement procedure

Resolution	8.000 - 2.780
R-factor	0.182
Rwork	0.182
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vam
RMSD bond length	0.008
RMSD bond angle	1.900
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000 *
High resolution limit [Å]	2.780 *	2.780 *
Rmerge	0.074	0.238 *
Total number of observations	151116 *
Number of reflections	37358
Completeness [%]	99.0	95.2 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	70 (mg/ml)
2	1	reservoir	Tris	10 (mM)
3	1	reservoir	ammonium sulfate	1 (M)