1CJE
ADRENODOXIN FROM BOVINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-01 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.443, 77.025, 59.681 |
| Unit cell angles | 90.00, 94.83, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.500 |
| Rwork | 0.233 |
| R-free | 0.31100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ayf |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.202 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.076 | 0.293 * |
| Total number of observations | 66064 * | |
| Number of reflections | 17178 * | |
| <I/σ(I)> | 27 | 3.9 |
| Completeness [%] | 92.0 | 57 * |
| Redundancy | 3.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 23 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 30 (%) | |
| 3 | 1 | reservoir | Na HEPES | 0.1 (M) | |
| 4 | 1 | reservoir | 150 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 40 (mM) |
