1CJA
ACTIN-FRAGMIN KINASE, CATALYTIC DOMAIN FROM PHYSARUM POLYCEPHALUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 178.900, 178.900, 59.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.27200 |
| Structure solution method | SIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.820 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.419 |
| Number of reflections | 23306 | |
| Completeness [%] | 94.9 | 77.4 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 7.5 * | 2.0 M LI2SO4, 0.1 M MES, PH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 2 | 1 | 1 | protein | 7.1 (mg/ml) | |
| 3 | 1 | 1 | AMP | 2.9 (mM) | |
| 4 | 1 | 1 | 0.6 (M) | ||
| 5 | 1 | 1 | MES/NaOH | 36 (mM) | |
| 6 | 1 | 2 | 2.0 (M) |
